In this exciting role, you will contribute to the production of transgenic, knockout, and knock-in mouse models. You will carry out various duties to support the transgenic mouse group and molecular biology lab as part of several projects aimed at identifying regulatory elements of importance in cardiovascular, limb, craniofacial, and brain development, and diseases. Specifically, you will generate transgenic/genome-edited mice and test evolving genome editing technologies (CRISPR/Cas9) via pronuclear microinjection of mouse eggs and surgical transfer of them into recipient mice. You will interact with the three Principal Investigators, the Transgenic Production Lead, postdoctoral fellows, and scientists to gain an understanding of their research needs. You will also be responsible for collecting, imaging, organizing, and providing preliminary analysis of the experimental data.
What You Will Do:
- Work with live research mice including husbandry and colony maintenance.
- Generate transgenic and knockout/knock-in mice by performing the following techniques:
- Pronuclear injections of mouse eggs using a microscope.
- surgically implanting embryos into pseudopregnant mice (small animal survival surgery).
- Inducing superovulation in mice by injecting hormones on weekdays and weekends.
- Identifying pseudo-pregnant recipients for embryo transfers by performing estrus cycle and vaginal plugs checks.
- Handling mouse eggs and blastocysts using a mouth-pipette.
- Contribute to all aspects of Transgenic Pipeline production including embryo production, egg recovery, and day-to-day operation and maintenance of the mouse colonies at the animal facility.
- Test genome editing tools to improve the efficiency of knockout and knock-in production.
- Purify, quantify, and prepare injection DNA mixes for pronuclear microinjection and other molecular biology tasks.
- Collect, fix, and stain post-implantation embryos as well as sort them for analysis and imaging.
- Assist with collection of mouse tissues by performing microdissection.
- Participate in ongoing research projects, analyze data and offer insight into the specifics of experimental results.
- Troubleshoot and optimize current standard operating protocols (SOPs) for transgenic/engineered mouse production to meet scientific research goals.
- Maintain an accurate and detailed laboratory notebook and updated databases of all experiments performed.
- Design experiments and perform preliminary analysis and interpret results.
Additional Responsibilities as needed:
- Maintain mouse lineages and perform general animal husbandry procedures (wean, ear tag).
- Inject hormones to superovulate egg donor mice.
- Collect zygotes from egg donors.
- Identify pseudo-pregnant recipients for embryo transfers by checking vaginal plugs.
- Genotype mice by isolating genomic DNA and performing PCR.
- Assist in mouse sperm cryopreservation and in vitro fertilization as part of the mouse program.
What is Required:
- B.A or B.S degree in Biology or a life science related field preferred and two to five years’ experience with increasing responsibility.
- Hands-on experience with mouse husbandry and colony management.
- Skilled in mouse handling including intraperitoneal and subcutaneous injections.
- Hands-on experience in performing pronuclear and blastocyst injections using a microscope.
- Proficient in performing survival surgical procedures required for embryo transfers.
- Ability to lift cages and move racks around in the animal facility.
- Knowledge and understanding of transgenic and knockout production technologies.
- Ability to perform basic molecular biology techniques including genomic DNA isolation and PCR.
- Detail oriented with excellent analytical, problem-solving, and troubleshooting skills.
- Demonstrated ability to work autonomously as well as part of a diverse team environment.
- Must possess strong organizational, writing and record-keeping skills including managing databases.
- Competent in the use of computers and software programs; i.e., Google Drive and Workspace applications, Microsoft Office, online laboratory notebooks.
- Experience in cryopreservation of mouse sperm and in vitro fertilization procedures.
- Background in genetics research.
- Willingness to work in a lab environment with changing priorities and technologies.
- Serve as a technical resource to more junior lab members regarding basic mouse husbandry.
- Experience developing Standard Operating Procedures related to duties and responsibilities.
- This is a full time, 1 year, term appointment with the possibility of extension or conversion to Career appointment based upon satisfactory job performance, continuing availability of funds and ongoing operational needs.
- This position is represented by a union for collective bargaining purposes.
- Salary will be determined based on range by collective bargaining agreement.
- This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.
- Diversity, equity, and inclusion are core values at Berkeley Lab. Our excellence can only be fully realized by faculty, students, and staff who share our commitment to these values. Successful candidates for our faculty positions will demonstrate evidence of a commitment to advancing equity and inclusion.
- Work will be primarily performed at Lawrence Berkeley National Lab, 1 Cyclotron Road, Berkeley, CA.
Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the Pay Transparency Nondiscrimination Provision under 41 CFR 60-1.4. Click
Lawrence Berkeley National Laboratory encourages applications from women, minorities, veterans, and other underrepresented groups presently considering scientific research careers.